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[Study of IL-6 signal transduction pathway in a human multiple myeloma cell line--Sko-007]
1Institute of Basic Medical Science, Academy of Military Medical Science, Beijing 100850, China.
Zhonghua Xue Ye Xue Za Zhi = Zhonghua Xueyexue Zazhi
|February 28, 2001
Objective:
To investigate the IL-6 signal transduction pathway in a human multiple myeloma (MM) cell line--Sko-007.
Methods:
The electrophoretic mobility shift assay (EMSA) and immunoprecipitation were used to detect the activation of IL-6-related transcription factors(TFs)--STAT3, NF-IL-6, NF-kB and protein kinase ERK, Jak1 in Sko-007 cells stimulated by IL-6.
Results:
Both the transcription factors-STAT3, NF-IL-6 and protein kinases-Jak1, ERK were activated by IL-6 in Sko-007 cells.
Conclusion:
The biological function of IL-6 on Sko-007 cells is mediated by the activation of Jak/STAT and Ras/NF-IL-6 signal transduction pathways.