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Digestion method for silver accumulated in micro-organisms.

R Joerger1, T Klaus, J Pettersson

  • 1Department of Materials Science, Uppsala University, Sweden. Ralph.Joerger@Angstrom.uu.se

Fresenius' Journal of Analytical Chemistry
|February 28, 2001
PubMed
Summary
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A new bomb digestion method effectively extracts silver from microbial samples. This technique allows for accurate silver quantification using inductively coupled plasma atomic emission spectroscopy.

Area of Science:

  • Environmental Science
  • Analytical Chemistry
  • Microbiology

Background:

  • Certain microbial strains accumulate high concentrations of silver.
  • Accurate determination of silver in biological matrices is crucial for environmental and health studies.

Purpose of the Study:

  • To develop and validate a novel method for silver extraction and digestion from organic matrices.
  • To enable quantitative analysis of silver in microbial samples.

Main Methods:

  • A bomb digestion technique utilizing nitric acid (HNO3) and hydrochloric acid (HCl) was employed.
  • The method was optimized for the complete extraction of silver and its compounds from complex organic matter.
  • Inductively coupled plasma atomic emission spectroscopy (ICP-AES) was used for quantitative silver determination.

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Main Results:

  • The bomb digestion method successfully extracted silver from various organic matrices.
  • Quantitative analysis of silver was achieved with high accuracy and precision.
  • The method demonstrated applicability for diverse microbial strains accumulating silver.

Conclusions:

  • The proposed bomb digestion method offers a reliable approach for silver analysis in microbial biomass.
  • This technique facilitates accurate assessment of silver accumulation in environmental and biological samples.
  • The method is suitable for routine quantitative determination of silver using ICP-AES.