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Apoptosis in rat erectile tissue induced by castration
1Research Center of Urology and Andrology, Hubei Medical University, Wuhan, China.
Aim:
To investigate the effect of androgen on the structure of corpus cavernosum.
Methods:
Thirty mature rats were randomized into 3 groups, i.e., simple castration, castration with testosterone (T) supplementation and sham-operated controls. One week after operation, the animals were sacrificed and corpora cavenosa harvested. Apoptosis was detected with the in situ end labeling (ISEL) techniques and DNA fragment analysis.
Results:
The apoptotic rate was 4.19% in the simple castrated rats, 0. 2% in castrated rats supplemented with T and 0.14% in the controls. Significant difference was found between the simple castrates and other two groups (P < 0.01). When comparing the T-supplementation group with the controls, there was no statistical difference (P > 0.05).
Conclusion:
Castration induced apoptosis in rat corpus cavernosum, that could be prevented by T supplementation. It suggests that androgen plays an important role in maintaining the structure of corpus cavernosum.