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Related Experiment Videos

Tuberization in potato involves a switch from apoplastic to symplastic phloem unloading.

R Viola1, A G Roberts, S Haupt

  • 1Unit of Plant Biochemistry, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, United Kingdom. rviola@scri.sari.ac.uk

The Plant Cell
|February 28, 2001
PubMed
Summary
This summary is machine-generated.

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Potato tuberization involves a shift from apoplastic to symplastic phloem unloading in stolons. This transition in sucrose transport is linked to changes in gene expression and invertase activity, regulating tuber development.

Area of Science:

  • Plant Physiology
  • Molecular Biology
  • Biochemistry

Background:

  • Phloem unloading is crucial for sink organ development in plants.
  • Tuberization in potato (Solanum tuberosum) involves complex physiological and metabolic changes.
  • Understanding assimilate partitioning is key to potato yield optimization.

Purpose of the Study:

  • To investigate the real-time dynamics of phloem unloading during early potato tuberization.
  • To compare phloem unloading patterns using phloem-mobile tracers and radiolabeled assimilates.
  • To elucidate the role of sucrose metabolism and invertase activity in tuber development.

Main Methods:

  • Real-time tracking of phloem unloading using carboxyfluorescein (CF) tracer.
  • Autoradiography of potato tubers after transport of (14)C assimilates.

Related Experiment Videos

  • Analysis of sugar levels, (14)C sugar partitioning, and invertase activity in stolons.
  • Histochemical analysis of reporter gene expression driven by an apoplastic invertase promoter.

    • Main Results:

    • Phloem unloading shifts from apoplastic to symplastic during early tuberization.
    • CF and (14)C assimilate transport patterns converge during the transition to symplastic unloading.
    • Soluble invertase declines in the subapical region, while cell wall-bound invertase remains high in the apical tuber region.
    • Apical and lateral tuber buds function as distinct metabolic domains.

    Conclusions:

    • The switch to symplastic sucrose unloading may upregulate sucrose metabolism genes.
    • Distinct invertase activities in apical and subapical regions suggest localized regulation of sucrose breakdown.
    • Tuber buds exhibit domain-specific sucrose unloading and metabolism, influencing potato development.