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Related Experiment Videos

Creatine kinase reactivation by thiol compounds.

D S Miyada, E C Dinovo, R M Nakamura

    Clinica Chimica Acta; International Journal of Clinical Chemistry
    |January 20, 1975
    PubMed
    Summary

    This study compares thiol activators for creatine kinase (CK) assays. Dithiothreitol (DTT) and mercaptoethanol showed varying effectiveness, impacting enzyme activity measurements.

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    Area of Science:

    • Biochemistry
    • Clinical Chemistry

    Background:

    • Thiol activated creatine kinase (CK) assay systems offer improved accuracy and reproducibility over non-activated systems.
    • Existing literature presents conflicting findings regarding the optimal thiol activators and their impact on CK enzyme activity.

    Purpose of the Study:

    • To investigate the relative effectiveness of different thiol compounds in reactivating serum CK.
    • To characterize the reaction kinetics of CK activation by various thiols.

    Main Methods:

    • Utilized the Oliver-Rosalki method for serum CK reactivation.
    • Compared the enzyme activities obtained using mercaptoethanol, cysteine, glutathione, and dithiothreitol (DTT).

    Main Results:

    • Significant variations in serum CK activity were observed depending on the specific thiol activator used.
    • Dithiothreitol (DTT) and mercaptoethanol demonstrated differential effects on enzyme activity compared to cysteine and glutathione.

    Conclusions:

    • The choice of thiol activator significantly influences the measured activity of serum creatine kinase.
    • Standardization of thiol activators is crucial for consistent and reliable CK assay results.

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