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Updated: Jul 28, 2026

Qualitative and Quantitative Assays for Detection and Characterization of Protein Antimicrobials
Published on: April 10, 2016
Antibacterial activity of a pepsin-derived bovine hemoglobin fragment
R Froidevaux1, F Krier, N Nedjar-Arroume
1Laboratoire de Technologie des Substances Naturelles, Institut Universitaire de Technologie, Département Génie Biologique, Université des Sciences et Technologie de Lille, PO Box 179, 59653 Villeneuve d'Ascq Cedex, France.
Abstract:
Peptic digestion of bovine hemoglobin yields a fragment with antibacterial activity. This peptide was purified to homogeneity by a two-step procedure including anion exchange chromatography and preparative reversed-phase HPLC. Mass determination and fragmentation indicated that this peptide corresponded to the 1-23 fragment of the alpha chain of hemoglobin. The minimum inhibitory concentration and mode of action of this peptide towards Micrococcus luteus strain A270 were determined. Hemolytic assay, interaction with liposomes, and study of its structure in solution were also performed.

