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Chromosome 13 abnormalities in multiple myeloma are mostly monosomy 13
H Avet-Louseau1, A Daviet, S Sauner
1Laboratory of Haematology, University Hospital, Nantes, France. havetloiseau@chu-nantes.fr
British Journal of Haematology
|March 3, 2001
Summary
Chromosome 13 abnormalities are common in multiple myeloma (MM), often indicating a poor prognosis. Fluorescence in situ hybridization (FISH) analysis targeting the 13q14 region is recommended for accurate diagnosis.
Area of Science:
- Genetics
- Oncology
- Cytogenetics
Background:
- Chromosome 13 abnormalities are prevalent in multiple myeloma (MM).
- These abnormalities are strongly linked to shorter patient survival.
- Complete monosomies represent the majority of observed chromosome 13 alterations.
Purpose of the Study:
- To identify the common minimal deletion region on chromosome 13 in MM patients.
- To evaluate the utility of fluorescence in situ hybridization (FISH) for detecting these abnormalities.
Main Methods:
- Analysis of 234 MM patients using FISH.
- Employing a panel of five probes targeting chromosome 13.
- Focusing on the 13q14 region and the D13S319 locus.
Main Results:
- Chromosome 13 abnormalities were detected in 42% of patients (98/234).
- Complete monosomy was present in 92% of these cases (90/98).
- Partial deletions primarily involved the 13q14 region (7/8 patients).
- A single patient (1%) showed a deletion at the D13S319 locus.
Conclusions:
- FISH is a valuable tool for analyzing chromosome 13 abnormalities in MM.
- Probes targeting the 13q14 region are crucial for detecting common deletions.