Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Gene expression analysis in colorectal cancer using practical DNA array filter.

K Okuno1, M Yasutomi, N Nishimura

  • 1The First Department of Surgery, Kinki University School of Medicine, Osaka-sayama, Japan.

Diseases of the Colon and Rectum
|March 3, 2001
PubMed
Summary

A new compact DNA array filter successfully identified unique gene expression patterns in colorectal cancer. This technology aids in classifying patients by molecular characteristics for future personalized treatments.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Paraneoplastic pemphigus associated with Castleman disease: progression from mucous to mucocutaneous lesions with epitope-spreading phenomena.

The British journal of dermatology·2017
Same author

Pulmonary embolism with myomatous erythrocytosis syndrome and extreme obesity.

The Thoracic and cardiovascular surgeon·2009
Same author

[Permanent pacemaker implantation after cardiac transplantation].

Herzschrittmachertherapie & Elektrophysiologie·2009
Same author

[Approaches and results in video-assisted thoracic surgery lobectomy for the patient with primary lung cancer].

Kyobu geka. The Japanese journal of thoracic surgery·2009
Same author

Comparison of postoperative surface light scattering of different intraocular lenses.

The British journal of ophthalmology·2009
Same author

Detectability of peripheral lung cancer on chest radiographs: effect of the size, location and extent of ground-glass opacity.

The British journal of radiology·2008

Area of Science:

  • Molecular Biology
  • Genomics
  • Cancer Research

Background:

  • Colorectal cancer (CRC) exhibits diverse gene expression profiles.
  • Accurate gene expression analysis is crucial for understanding CRC pathogenesis.
  • Novel tools are needed for efficient and detailed gene expression profiling in individual tumors.

Purpose of the Study:

  • To evaluate the utility of a novel, compact DNA array filter for analyzing gene expression in individual colorectal cancer samples.
  • To establish a method for characterizing molecular differences between cancerous and normal colorectal tissues.

Main Methods:

  • Complementary DNA (cDNA) probes were synthesized from mRNA of colorectal cancer and normal mucosa specimens.
  • Chemiluminescence labeling was used for probe detection on the DNA array filter.

Related Experiment Videos

  • A specialized charge-coupled device camera and computer software quantified gene expression levels based on signal intensity.

    • Main Results:

    • The compact DNA array filter successfully generated characteristic gene expression patterns from cell lines (RPMI4788, HL60).
    • Key gene expression changes in colorectal cancer included up-regulation of nm23, TIMP1, VEGF, and cyclin E.
    • Down-regulation of tumor suppressor genes (p53, TOSO, SIVA), beta-catenin, and metallothionein was observed in cancer tissues compared to normal tissues.

    Conclusions:

    • The developed compact DNA array filter system effectively captures unique gene expression patterns in colorectal cancer.
    • This technology provides a foundation for collecting and analyzing extensive gene expression data.
    • Future analysis of large datasets will facilitate the identification of molecular subtypes within colorectal cancer patient populations.