Related Experiment Video
Updated: Jul 28, 2026
08:44
Generation of Topically Transgenic Rats by In utero Electroporation and In vivo Bioluminescence Screening
Published on: September 24, 2013
Generation of a high-density rat EST map
T E Scheetz1, M R Raymond, D Y Nishimura
1Howard Hughes Medical Institute, University of Iowa, Iowa City, Iowa 52242, USA.
Genome Research
|March 7, 2001
Summary
Researchers created a high-density rat gene map using over 11,000 expressed sequence tags (ESTs). This valuable resource aids in identifying genes for diseases and advancing rat genome sequencing efforts.
Area of Science:
- Genomics
- Comparative Genomics
- Genetic Mapping
Background:
- High-density genetic maps are crucial for gene discovery and understanding genome organization.
- The rat is a key model organism for studying human diseases.
Purpose of the Study:
- To develop a high-density expressed sequence tag (EST) map for the rat.
- To facilitate comparative mapping between rat, human, and mouse genomes.
- To support positional cloning of disease genes and rat genome sequencing.
Main Methods:
- Development of a radiation hybrid (RH) framework map covering all rat chromosomes.
- Mapping over 11,000 ESTs onto the RH framework map.
- Duplicate mapping of ESTs and consensus RH vector generation to minimize errors.
- Construction of high-density comparative maps using the rat EST map.
Main Results:
- A high-density rat EST map comprising >11,000 ESTs was successfully developed.
- The framework map spans approximately 12,400 centiRays (cR) with an average resolution of 240 kb/cR.
- Comparative maps between rat, human, and mouse genomes were constructed.
Conclusions:
- The developed rat EST map is a significant resource for genetic research.
- This map will accelerate positional cloning of genes in rat models of human diseases.
- It will aid in the creation and verification of map order for rat genome sequencing projects.
