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Reciprocal consomic strains to evaluate y chromosome effects
C D Negrín1, M W McBride, H V Carswell
1Department of Medicine and Therapeutics, University of Glasgow, Western Infirmary, and Wellcome Surgical Institute University of Glasgow, United Kingdom.
Hypertension (Dallas, Tex. : 1979)
|March 7, 2001
Summary
The SHRSP Y chromosome influences systolic blood pressure (BP) but not vulnerability to cerebral ischemia. Transferring the WKY Y chromosome lowered BP in SHRSP rats, while the SHRSP Y chromosome increased BP in WKY rats.
Area of Science:
- Genetics
- Cardiovascular Science
- Neuroscience
Background:
- Previous studies indicated the SHRSP Y chromosome contributes to hypertension and increased vulnerability to cerebral ischemia.
- A codominant inheritance pattern suggested a potential role for the Y chromosome in these traits.
Purpose of the Study:
- To investigate the role of the Y chromosome in blood pressure (BP) regulation.
- To determine the Y chromosome's contribution to vulnerability to cerebral ischemia.
Main Methods:
- Construction of consomic rat strains by exchanging Y chromosomes between SHRSP and WKY rats.
- Measurement of systolic blood pressure via radiotelemetry during baseline and salt-loading periods.
- Induction of permanent middle cerebral artery occlusion (MCAO) to assess infarct volume.
Main Results:
- Transferring the WKY Y chromosome to SHRSP significantly reduced systolic BP during salt loading.
- The SHRSP Y chromosome significantly increased systolic BP in WKY rats during baseline.
- No significant differences in infarct volumes were observed between consomic strains and their parental strains.
Conclusions:
- The SHRSP Y chromosome harbors a locus that contributes to systolic BP regulation.
- The Y chromosome does not appear to contribute to the vulnerability to focal cerebral ischemia in this model.