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Effect of errors in the sequence of optical densities from the Roche AMPLICOR HIV-1 MONITOR assay on the validity of
D J Brambilla1, S Granger, C Jennings
1New England Research Institutes, Inc., Watertown, Massachusetts 02472, USA. donb@neri.org
Journal of Clinical Microbiology
|March 7, 2001
Abstract:
Specifications for the AMPLICOR HIV-1 MONITOR kit indicate that the results are invalid if the optical densities (ODs) from the PCR-amplified sample that are between 0.1 and 2.3 units are out of sequence. However, among 11,904 assays, results were biased only when ODs of 0.2 to 2.0 units were out of sequence, reducing the rate of invalid results from 3.2 to 0.59%.