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Published on: December 3, 2011
Quantitation of porcine cytomegalovirus in pig tissues by PCR
J F Fryer1, P D Griffiths, J A Fishman
1Department of Virology, Royal Free and University College Medical School of UCL, Rowland Hill Street, London NW3 2PF, United Kingdom.
Journal of Clinical Microbiology
|March 7, 2001
Abstract:
A quantitative-competitive PCR for the quantification of porcine cytomegalovirus (PCMV) was developed. The virus was detected in a variety of pig organs (including potential xenotransplant donations), with viral loads ranging from <10 to 97 genome copies/microg of DNA. This assay will have significant utility for studying the activation and replication of PCMV and in swine models for allo- and xenotransplantation.
