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Related Experiment Videos

In vitro decrease of glomerular heparan sulfate by lymphocytes from idiopathic nephrotic syndrome patients.

B Birmele1, G Thibault, H Nivet

  • 1Department of Pediatrics, University Hospital of Geneva, Geneva, Switzerland.

Kidney International
|March 7, 2001
PubMed
Summary

Peripheral blood lymphocytes from patients with idiopathic nephrotic syndrome (INS) significantly reduce glomerular cell glycosaminoglycans (GAGs), including heparan sulfates (HS). This finding suggests a direct role for lymphocytes in the development of albuminuria in INS.

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Area of Science:

  • Nephrology
  • Immunology
  • Cell Biology

Background:

  • Lymphocytes play a role in idiopathic nephrotic syndrome (INS) pathogenesis.
  • Previous studies showed INS patient plasma reduces glomerular epithelial cell (GEC) glycosaminoglycans (GAGs), especially heparan sulfates (HS).
  • This study investigates the impact of peripheral blood lymphocytes (PBL) from INS patients on GEC GAG and HS.

Purpose of the Study:

  • To determine if peripheral blood lymphocytes (PBL) from idiopathic nephrotic syndrome (INS) patients affect glomerular cell glycosaminoglycans (GAGs) and heparan sulfates (HS).
  • To compare the effects of lymphocytes from INS patients versus healthy controls on GEC GAG and HS production.

Main Methods:

  • Human GECs were co-cultured with peripheral blood mononuclear cells (PBMCs), PBLs, or monocytes from INS patients and controls.

Related Experiment Videos

  • GAG and HS levels were quantified using metabolic labeling and a cationic membrane assay.

    • Main Results:

    • Mononuclear cells from controls reduced GEC GAGs and HSs.
    • PBLs from INS patients caused a significantly greater decrease in GEC GAGs and HSs compared to control PBLs.
    • Supernatants from stimulated PBMCs of INS patients also reduced GAG and HS levels more than controls.

    Conclusions:

    • Peripheral blood lymphocytes (PBLs) from INS patients directly decrease glomerular epithelial cell (GEC) heparan sulfates (HS).
    • This lymphocyte-mediated reduction in HS may contribute to the development of albuminuria in idiopathic nephrotic syndrome (INS).