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Sperm function tests after vasovasostomy.

R Q Wen1, M Y Lie, P Tian

  • 1Family Planning Research Institute of Guangdong, Guangzhou, China. wenrq@163.net

Asian Journal of Andrology
|March 10, 2001
PubMed
Summary
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Sperm function, including hypoosmotic swelling test (HOST) and acrosome reaction, shows reduced levels after vasectomy reversal, gradually recovering over time. Acridine orange (AO) fluorescence indicates no genetic material damage from vasovasostomy.

Area of Science:

  • Reproductive Medicine
  • Andrology
  • Urology

Background:

  • Vasovasostomy is a surgical procedure for vasectomy reversal.
  • Assessing sperm function post-vasovasostomy is crucial for evaluating surgical success and potential fertility restoration.

Purpose of the Study:

  • To evaluate and compare sperm function parameters in men following vasovasostomy at different time intervals.
  • To determine the recovery pattern of sperm function after vasectomy reversal.

Main Methods:

  • Semen samples were collected from 42 men post-vasovasostomy (categorized by time post-reversal) and 34 fertile controls.
  • Sperm function was assessed using hypoosmotic swelling test (HOST), acridine orange (AO) fluorescence, triple-stain for acrosome reaction, and cervical mucus penetration test (CMPT).

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Main Results:

  • Hypoosmotic swelling test (HOST) percentages were significantly lower in vasovasostomy groups compared to controls, with gradual recovery observed.
  • Acrosome reaction rates were significantly lower in the early post-reversal group (1-6 months) but normalized by 6-12 months.
  • Acridine orange (AO) fluorescence showed no significant differences, suggesting intact sperm DNA.
  • Cervical mucus penetration test (CMPT) revealed higher rates of abnormal ('poor' and 'negative') results in early and late post-reversal groups.

Conclusions:

  • Sperm membrane integrity (HOST) remains compromised for up to a year post-vasovasostomy, with recovery thereafter.
  • Acrosome reaction potential can normalize within six months after vasectomy reversal.
  • Vasovasostomy does not appear to affect sperm DNA integrity (AO data).
  • Comprehensive evaluation of cervical mucus penetration test (CMPT) results, including abnormal types, is necessary for assessing vasectomy reversal outcomes.