Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Comparative study on efficacy of three sperm-separation techniques.

L Xu1, R K Lu, L Chen

  • 1Reproduction Center, Dept. of Gynec/Obstet, First Affiliated Hospital, Shantou University Medical College, China. cysxl@pub.shantou.gd.cn

Asian Journal of Andrology
|March 10, 2001
PubMed
Summary

The real-time sperm separation technique, Wang's tube method, yields superior sperm quality and higher pregnancy rates in intrauterine insemination (IUI) compared to conventional methods. This advanced technique effectively removes impurities for improved assisted reproduction outcomes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Covalent linkage between proteins of the inter-alpha-inhibitor family and hyaluronic acid is mediated by a factor produced by granulosa cells.

The Journal of biological chemistry·1996
Same author

Two-tone rate suppression boundaries of cochlear ganglion neurons in normal chickens.

The Journal of the Acoustical Society of America·1996
Same author

Greater ozone-induced inflammatory responses in subjects with asthma.

American journal of respiratory and critical care medicine·1996
Same author

Use of combinatorial peptide libraries to construct functional mimics of tumor epitopes recognized by MHC class I-restricted cytolytic T lymphocytes.

The Journal of experimental medicine·1996
Same author

Recurrent transition at a CG dinucleotide in exon 12 of COL2A1 produces kniest dysplasia with abnormal RNA splicing by chondrocytes and lymphoblasts and interruption of the triple helix of type II collagen.

Annals of the New York Academy of Sciences·1996
Same author

Inogatran, a novel direct low molecular weight thrombin inhibitor, given with, but not after, tissue-plasminogen activator, improves thrombolysis.

The Journal of pharmacology and experimental therapeutics·1996

Area of Science:

  • Reproductive Medicine
  • Sperm Biology
  • Assisted Reproductive Technology

Background:

  • Sperm preparation is critical for successful assisted reproduction.
  • Conventional methods like swim-up and density gradient may have limitations in isolating high-quality sperm and removing contaminants.
  • Evaluating novel techniques is essential for improving outcomes.

Purpose of the Study:

  • To compare the effectiveness of the real-time sperm separation technique (Wang's tube method) against two conventional methods.
  • To assess the quality of sperm preparations obtained by each method.
  • To compare intrauterine insemination (IUI) pregnancy rates using sperm prepared by the different techniques.

Main Methods:

  • Sperm samples from 60 infertile patients were processed using Wang's tube method, swim-up, and Percoll density gradient.

Related Experiment Videos

  • Semen quality parameters, including morphology, chromatin integrity, and motility, were evaluated.
  • IUI pregnancy rates were compared in 80 oligoasthenoteratospermic patients.

    • Main Results:

    • The Wang's tube method significantly improved sperm quality (P < 0.01) compared to conventional methods.
    • This technique was more effective in removing microorganisms and impurities.
    • A higher IUI pregnancy rate was observed with sperm prepared using the Wang's tube method (P < 0.05).

    Conclusions:

    • The real-time sperm separation technique (Wang's tube method) is highly effective for isolating high-quality sperm.
    • This method offers superior sperm preparation for assisted reproduction.
    • It leads to improved pregnancy outcomes in IUI procedures.