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DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis
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High-throughput AFLP analysis using infrared dye-labeled primers and an automated DNA sequencer

A A Myburg1, D L Remington, D M O'Malley

  • 1Forest Biotechnology Group, Box 7247, Centennial Campus, North Carolina State University, Raleigh, NC 27695, USA. aamyburg@unity.ncsu.edu

Biotechniques
|March 10, 2001
PubMed
Summary

This study introduces a high-throughput protocol for Amplified Fragment Length Polymorphism (AFLP) analysis, enabling rapid generation of numerous DNA markers. The enhanced method achieves up to 70,000 genotype determinations weekly, significantly advancing genomic research.

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