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Sensitive measurement of polyethylene glycol-modified proteins
N M Tsai1, T L Cheng, S R Roffler
1Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan.
Biotechniques
|March 10, 2001
Abstract:
An IgM monoclonal antibody (AGP3) against polyethylene glycol (PEG) was used to assay PEG-modified proteins by ELISA. PEG-modified beta-glucuronidase could be measured at concentrations as low as 15 ng/mL, corresponding to 750 pg (1.8 fmol) of conjugate. This ELISA should be generally applicable to all PEG-modified proteins because AGP3 binds the backbone of the PEG chain independent of the linker used for PEG attachment.