[Studies of multidrug resistance gene expression and apoptosis in stomach carcinoma]
Objective:
To investigate the influence of multidrug resistance gene (MDR) expression and apoptosis on stomach cancer.
Methods:
A total of 80 cases of paraffin embedded stomach cancer tissues was stained immunohistochemically for P-gp, GST-pi and TOPO II using monoclonal antibodies and apoptosis (in terms of apoptotic index, AI) in tissues was musured by the TUNEL method. The results were correlated with tumor type, clinical stage and prognosis.
Results:
Normal stomach tissue expression of P-gp was associated with better survival, but higher level of P-gp expression in tumor tissues was associated with poor prognosis. The expression of GST-pi didnot show a relationship with cell differentiation of stomach cancer and clinical stage of the disease, but positive correlation did exist between intensity of expression and survival time. Although the expression of TOPO II was associated with tumor cell differentiation, it did not affect prognosis. Apoptosis was demonstrated in 73% of the cases and was related to clinical stage of stomach carcinoma.
Conclusion:
While P-gp expression in tumor cells is indicative of poor prognosis, its expression in normal tissue has the opposite effect. Positive correlation exits between GST-pi expression intensity in tumor cells and survival. MDR and apoptosis are related to the biologic behavior of the tumor.
Insights
Multidrug resistance (MDR) gene expression and apoptosis influence stomach cancer. P-glycoprotein (P-gp) in tumors indicates poor prognosis, while GST-pi intensity correlates with survival, impacting tumor behavior.
Area of Science:
- Oncology
- Molecular Biology
- Cancer Research
Background:
- Stomach cancer prognosis is influenced by multidrug resistance (MDR) gene expression and apoptosis.
- Understanding these factors is crucial for improving patient outcomes.
Purpose of the Study:
- To investigate the role of MDR gene expression (P-gp, GST-pi, TOPO II) and apoptosis in stomach cancer.
- To correlate these markers with tumor characteristics and patient prognosis.
Main Methods:
- Immunohistochemical staining of 80 stomach cancer tissues for P-gp, GST-pi, and TOPO II.
- TUNEL method to measure apoptotic index (AI).
- Correlation analysis with tumor type, clinical stage, and survival.
Main Results:
- P-gp expression in normal tissue associated with better survival; in tumor tissue, associated with poor prognosis.
- GST-pi expression intensity positively correlated with survival time.
- TOPO II expression linked to tumor differentiation but not prognosis.
- Apoptosis observed in 73% of cases, related to clinical stage.
Conclusions:
- P-gp expression in tumor cells indicates poor prognosis, contrasting with its effect in normal tissue.
- GST-pi expression intensity is a positive prognostic marker.
- MDR and apoptosis are integral to the biological behavior of stomach tumors.
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