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Updated: May 3, 2026

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion

Published on: June 25, 2017

A chiroselective peptide replicator.

A Saghatelian¹, Y Yokobayashi, K Soltani

¹Department of Chemistry, and Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, California 92037, USA.

|March 10, 2001

Summary

This summary is machine-generated.

Scientists created a self-replicating peptide that amplifies homochiral molecules from a racemic mixture. This peptide replicator demonstrates a novel chiroselective editing function, supporting the role of polypeptides in the origin of homochirality.

Keywords:

NASA Discipline Exobiology Non-NASA Center

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Identification of Functional Protein Regions Through Chimeric Protein Construction

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Published on: January 8, 2019

Area of Science:

Origin of Life Studies
Biochemistry
Chemical Evolution

Background:

The origin of homochirality (the predominance of one enantiomer) in biological systems remains a significant puzzle.
Existing physicochemical models struggle to explain the substantial enantiomeric imbalance required for homochiral biopolymers.
Chiroselective molecular replication is proposed as a key mechanism for amplifying initial enantiomeric differences.

Purpose of the Study:

To investigate the potential of nonenzymatic, self-replicating peptides in generating homochirality.
To explore mechanisms for amplifying enantiomeric excess in prebiotic chemistry.
To provide experimental support for the role of self-replicating polypeptides in the origin of homochirality on Earth.

Main Methods:

Design and synthesis of a 32-residue peptide replicator based on established principles.
Experimental demonstration of chiroselective autocatalysis using racemic peptide fragments.
Analysis of the system's ability to amplify homochiral products and its stereochemical discrimination capabilities.

Main Results:

The designed peptide replicator efficiently amplifies homochiral products from a racemic mixture via a chiroselective autocatalytic cycle.
The system exhibits high fidelity, discriminating against sequences with even single stereochemical mutations.
A dynamic stereochemical 'editing' function was observed, utilizing heterochiral byproducts to enhance homochiral product formation.

Conclusions:

Self-replicating polypeptides can effectively amplify homochirality from racemic mixtures.
The observed chiroselective amplification and editing mechanisms provide a plausible pathway for the origin of homochirality.
These findings strengthen the hypothesis that self-replicating peptides played a crucial role in early life's homochiral nature.