Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Prospects for ab initio protein structural genomics.

K T Simons¹, C Strauss, D Baker

¹Unversity of Washington, Seattle, WA 98195, USA.

Journal of Molecular Biology

|March 10, 2001

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Psychological factors and long-term tinnitus handicap in vestibular schwannoma patients after retrosigmoid microsurgery - a cross-sectional study.

International journal of audiology·2024

Same author

[Thoracic calcifications].

La Revue de medecine interne·2023

Same author

[Pulmonary phenotypes of inborn errors of metabolism].

Revue des maladies respiratoires·2022

Same author

Validation of the Hamilton Program for Schizophrenia Voices Questionnaire: Associations with emotional distress and wellbeing, and invariance across diagnosis and sex.

Schizophrenia research·2021

Same author

Management of transmodiolar and transmacular cochleovestibular schwannomas with and without cochlear implantation.

HNO·2020

Same author

[Hearing classification in patients with vestibular schwannoma using German-language test procedures].

HNO·2020

Same journal

UPF3A and UPF3B shape the transcriptome cooperatively yet oppose cell function.

Journal of molecular biology·2026

Same journal

Antibody-secreting cells integrate efficient NMD with non‑canonical UPR signaling to maintain proteostasis and support massive immunoglobulin synthesis.

Journal of molecular biology·2026

Same journal

Small molecule stabilization of diverse amyloidogenic immunoglobulin light chains revealed by hydrogen-deuterium exchange mass spectrometry.

Journal of molecular biology·2026

Same journal

UPF1 at Work: Structural and Mechanistic Insights Into a Master Regulator of Nonsense-Mediated mRNA Decay.

Journal of molecular biology·2026

Same journal

Structural basis for the pro-amyloidogenic action and ligand binding of a novel W72R variant of human apolipoprotein A-I.

Journal of molecular biology·2026

Same journal

Cryo-EM Structure of the C. Elegans Septin Tetramer Reveals a Revised Architecture and Conserved Positional Orthology.

Journal of molecular biology·2026

See all related articles

The ROSETTA method successfully predicted structures for many small proteins using ab initio protein structure prediction. This advance aids in identifying related protein structures and functions genome-wide.

Area of Science:

Computational Biology
Structural Biology
Bioinformatics

Background:

Accurate protein structure prediction is crucial for understanding biological function.
Ab initio methods aim to predict protein structures from sequence alone, offering a powerful alternative to experimental methods.

Purpose of the Study:

To evaluate the performance of the ROSETTA method for ab initio protein structure prediction on a large scale.
To assess the accuracy of predicted models using traditional and structure-based comparison metrics.

Main Methods:

Generated protein models for small proteins (up to 150 amino acids) using the ROSETTA method.
Evaluated models using root-mean-square deviation (rmsd) and DALI structure-based comparisons against the Protein Data Bank (PDB).

Related Experiment Videos

Main Results:

The ROSETTA method produced at least one model within 7 Å rmsd of the native structure for 111 out of 136 proteins (50-150 residues).
For 60 proteins, the top predicted conformations showed structurally related matches in the PDB to the native protein.

Conclusions:

Ab initio protein structure prediction methods show promise for generating low-resolution models.
These approaches may facilitate the identification of distantly related proteins with similar structures and functions on a genomic scale.