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Published on: February 6, 2018
Purification of a fragment of MDM2 for production of polyclonal antisera
1Department of Oncology, McArdle Laboratory for Cancer Research, Madison, Wisconsin 53706, USA.
Protein Expression and Purification
|March 10, 2001
Abstract:
A histidine-tagged, carboxy-terminal fragment of the murine double minute 2 gene product, p90(MDM2), was purified by Ni--NTA chromatography and preparative gel electrophoresis. The purified MDM2 fragment was used to generate polyclonal antisera that recognize multiple species of MDM2 proteins, including the inhibitor of p53, p90(MDM2), as well as the activator of p53, p76(MDM2). The antibodies are useful for Western analysis, immunoprecipitation, and immunofluorescence.
