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Updated: Aug 8, 2026

Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes
Published on: October 23, 2014
In vivo chromatin remodeling by yeast ISWI homologs Isw1p and Isw2p
N A Kent1, N Karabetsou, P K Politis
1Division of Molecular Genetics, Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK. nakent@molbiol.ox.ac.uk
Abstract:
Isw1p and Isw2p are budding yeast homologs of the Drosophila ISWI chromatin-remodeling ATPase. Using indirect-end-label and chromatin immunoprecipitation analysis, we show both independent and cooperative Isw1p- and Isw2p-mediated positioning of short nucleosome arrays in gene-regulatory elements at a variety of transcription units in vivo. We present evidence that both yeast ISWI complexes regulate developmental responses to starvation and that for Isw2p, recruitment by different DNA-binding proteins controls meiosis and haploid invasive growth.
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