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Physical and functional interactions between Zic and Gli proteins.
Y Koyabu1, K Nakata, K Mizugishi
1Laboratory for Developmental Neurobiology, RIKEN Brain Science Institute, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.
The Journal of Biological Chemistry
|March 10, 2001
Summary
Zic and Gli proteins physically interact, with Zic proteins influencing Gli
Area of Science:
- Developmental biology
- Molecular biology
- Genetics
Background:
- Zic and Gli proteins are transcription factors with similar zinc finger domains.
- Previous research suggests Zic and Gli collaborate in neural and skeletal development.
Purpose of the Study:
- To investigate the physical and functional interaction between Zic and Gli proteins.
- To elucidate the role of Zic proteins in modulating Gli protein activity and the hedgehog signaling pathway.
Main Methods:
- Co-expression studies to observe protein interactions.
- Analysis of protein translocation to the cell nucleus.
- Assessment of transcriptional activity regulation.
Main Results:
- Zic and Gli proteins physically interact via their zinc finger domains.
- Zic proteins facilitate the nuclear translocation of Gli proteins.
- Both Zic and Gli proteins mutually regulate each other's transcriptional activity.
Conclusions:
- Physical interaction between Zic and Gli underlies their developmental roles.
- Zic proteins act as potential modulators of the hedgehog signaling pathway.