Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Testing time-, ignorance-, and danger-based models of tolerance.

C C Anderson1, J M Carroll, S Gallucci

  • 1Ghost Lab, Section on T cell Tolerance and Memory, Laboratory of Cellular and Molecular Immunology, National Institute of Allergy and Infectious Diseases/National Institutes of Health, Bethesda, MD 20892, USA. canderson@niaid.nih.gov

Journal of Immunology (Baltimore, Md. : 1950)
|March 10, 2001
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Computational Evaluation of Combined Cerebellar and Frontal Transcranial Direct Current Stimulation for Treatment-Resistant Depression.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2022
Same author

Gold nanoparticles as enablers of cell membrane permeabilization by time-varying magnetic field: influence of distance and geometry.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2022
Same author

Application of Stochastic Dosimetry for assessing the Human RFEMF Exposure in a 5G indoor Scenario.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2021
Same author

Contactless Cell Permeabilization by Time-Varying Magnetic fields: Modelling Transmembrane Potential and Mechanical Stress in in- vitro Experimental Set-Up.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2021
Same author

Cell transmembrane potential in contactless permeabilization by time-varying magnetic fields.

Computers in biology and medicine·2021
Same author

Does the Danger model shed any light on central tolerance?: A response to Al-Yassin.

Scandinavian journal of immunology·2018

Peripheral tissue acceptance is not due to timing or newborn immune system properties. Newly developing T cells reject male grafts, indicating immune response is context-dependent, not based on antigen (Ag) appearance time.

Area of Science:

  • Immunology
  • Transplantation Biology

Background:

  • Peripheral tolerance mechanisms are not fully understood.
  • The role of antigen (Ag) timing and neonatal immune system properties in tolerance is debated.

Purpose of the Study:

  • To investigate the factors determining peripheral tissue acceptance versus rejection.
  • To determine if tolerance is influenced by the timing of Ag exposure or neonatal tissue characteristics.

Main Methods:

  • Grafting male tissues onto immunoincompetent female mice, followed by repopulation with fetal liver stem cells.
  • Analyzing T cell response to established grafts.
  • Assessing H-Y antigen cross-presentation on host antigen-presenting cells (APCs).
  • Quantifying mRNA expression of stress/inflammatory molecules in grafts using real-time quantitative PCR.

Related Experiment Videos

Main Results:

  • Newly generated T cells rejected male grafts, demonstrating active immune response, not tolerance or ignorance.
  • H-Y antigen cross-presentation on host APCs was immunogenic and graft-dependent.
  • Healed grafts showed lower GAPDH expression compared to normal skin, suggesting altered tissue state.

Conclusions:

  • Peripheral tissue acceptance is not determined by Ag appearance timing, neonatal properties, or naive T cell circulation.
  • Immune response to peripheral tissues likely depends on unidentified aspects of the antigen presentation context.
  • The findings challenge existing models of immune tolerance and highlight the importance of the microenvironment in immune responses.