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Updated: Aug 9, 2026

Electrochemiluminescence Assays for Human Islet Autoantibodies
Published on: March 23, 2018
Hypersialylated macromolecular serum immunoglobulin A1 in type 2 diabetes mellitus
L Vázquez-Moreno1, M C Candia-Plata, M R Robles-Burgueño
1Centro de Investigación en Alimentación y Desarrollo, A.C. Apartado Postal 1735, Hermosillo, 83,000, Sonora, Mexico. lvazguez@cascabel.ciad.mx
Objectives:
The origin of the elevation of serum immunoglobulin A1 (IgA1) in Type 2 diabetes mellitus (DM) is unsettled. The aim of this study was to address the carbohydrate changes of serum IgA1 from patients with Type 2 diabetes mellitus, as a possible cause of the elevation.
Design And Methods:
IgA1 was purified from sera of 6 DM patients and 4 healthy matched controls by using highly acetylated-Sepharose 6B, anti-IgA-agarose, and jacalin-agarose columns, and further separated into jacalin low-affinity, medium, and high-affinity fractions. Hinge and Fc fragments from native IgA1 were obtained and analyzed by using Sambucus nigra, Maackia amurensis, Arachis hypogaea, Erythrina cristagalli, and Ricinus communis lectins.
Results:
The jacalin high-affinity fraction, mostly constituted by macromolecular IgA1, was more abundant in DM patients than in controls and also more reactive to Sambucus nigra, and Maackia amurensis lectins.
Conclusions:
Macromolecular serum IgA1 from DM patients is hypersialylated and this probably contributes to the high level of IgA1 in DM patients.
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