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Updated: Aug 2, 2026

Characterization of G Protein-coupled Receptors by a Fluorescence-based Calcium Mobilization Assay
11:49

Characterization of G Protein-coupled Receptors by a Fluorescence-based Calcium Mobilization Assay

Published on: July 28, 2014

Orphan G-protein-coupled receptors and natural ligand discovery

A D Howard1, G McAllister, S D Feighner

  • 1Dept of Metabolic Disorders, Merck Research Laboratories, PO Box 2000, Rahway, NJ 07065, USA. andrew_howard@merck.com

Trends in Pharmacological Sciences
|March 10, 2001
PubMed

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G-protein Coupled Receptors

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G protein-coupled receptor (GPCR) signaling plays a crucial role in cell functioning. GPCR desensitization is an equally essential process. It allows cells to respond to changing environments and regain sensitivity to new stimuli while preventing unnecessary stimulation when no longer needed. Prolonged exposure to stimuli leads to GPCR desensitization. It involves blocking the receptors from binding and activating additional G proteins. This inhibits activation of downstream effectors, thereby...
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