Centrifuge polarizing microscope. II. Sample biological applications
1Marine Biological Laboratory, Woods Hole, MA 02543, U.S.A.
Abstract:
The rationale, design and general performance of the CPM (centrifuge polarizing microscope) were described in Part I of this study (Inoué et al. J. Microsc. 201 (2001) 341-356. In this second part, we describe observations on several biological samples that we have explored over the past two years using the CPM. As described in the first part of the study, although the CPM was basically designed as a high-extinction centrifuge polarizing microscope, it also allows observations of the specimen exposed to high centrifugal fields up to 10 500 x g (earth's gravitational acceleration) in fluorescence (532-nm excitation) and in DIC (differential interference or Nomarski contrast).
More Related Videos
Related Concept Videos
Overview Of Cell Separation And Isolation
Subcellular Fractionation
Differential Centrifugation
Differential centrifugation is...
Imaging Biological Samples with Optical Microscopy
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Fixation and Sectioning
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
Centrifugation
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...


