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A simple and efficient method to transcribe RNAs with reduced 3' heterogeneity

C Kao1, S Rüdisser, M Zheng

  • 1Department of Biology, Indiana University, Bloomington, Indiana 47405, USA. ckao@bio.indiana.edu

Methods (San Diego, Calif.)
|March 13, 2001
PubMed

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RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...