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Author Spotlight: Improved Methods for Preparing Transverse Sections and Unrolled Whole Mounts of Maize Leaf Primordia for Fluorescence and Confocal Imaging

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Mapping maize sequences to chromosomes using oat-maize chromosome addition materials.

R J Okagaki¹, R G Kynast, S M Livingston

¹Department of Agronomy and Plant Genetics, United States Department of Agriculture, Agricultural Research Service, University of Minnesota, St. Paul, MN 55108, USA. okaga002@tc.umn.edu

Plant Physiology

|March 13, 2001

Summary

Researchers mapped 400 maize sequences to chromosomes using oat-maize addition lines. This provides valuable positional information for identifying important maize genes.

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Area of Science:

Plant genetics
Genomics
Molecular biology

Background:

Oat-maize chromosome additions facilitate the study of maize chromosomes.
Each of the 10 maize chromosomes has been isolated in oat.
This material enables the mapping of maize sequences to specific chromosomes.

Purpose of the Study:

To map 400 maize DNA sequences to specific chromosomes using oat-maize addition lines.
To validate mapping results against previously mapped cloned markers.
To map previously unmapped expressed sequence tags (ESTs) and sequence tagged sites (STSs).

Main Methods:

Utilizing polymerase chain reaction (PCR) and DNA from oat-maize addition lines.
Employing Southern-blot analysis for validation of mapped cloned markers.
High-throughput mapping strategies.

Main Results:

Successfully mapped 400 maize sequences to chromosomes.
Mapping results for 50 cloned markers were consistent with previous linkage analysis and Southern-blot data.
Mapped 350 previously unmapped ESTs and STSs to specific maize chromosomes.

Conclusions:

Oat-maize addition lines are effective tools for high-throughput mapping of maize sequences.
The generated positional information aids in identifying candidate genes.
This approach accelerates the understanding of maize gene function and organization.