A confocal microscopic study of synaptic inputs to gonadotropin-releasing hormone cells in mouse brain: regional
1Division of Endocrinology and Arthur Fishberg Center for Neurobiology, Mount Sinai Medical Center, New York, NY, USA. rajeng01@doc.mssm.edu
Abstract:
Even though the cells producing gonadotropin-releasing hormone (GnRH) are scattered in the basal forebrain, a large proportion of them is present in the organum vasculosum of the lamina terminalis (OVLT) and in the preoptic area. The present studies were undertaken to investigate whether there is any difference in the number of synaptic inputs between GnRH cells located in the OVLT and those located at more anterior levels of the brain. Immunohistochemical staining for the synaptic marker synaptophysin coupled with confocal microscopy was employed to analyze synaptic inputs to GnRH cells located at the two levels examined. The results indicate that GnRH cells in the OVLT region receive a greater number of synaptophysin-immunoreactive appositions as compared with those located in the anterior septum. This supports the existence of subsets among the GnRH cells located in the basal forebrain. The effect of estradiol on the number of synaptophysin-immunoreactive appositions onto GnRH cells was also studied. Treatment of ovariectomized mice with estradiol significantly enhanced the number of synaptophysin-immunoreactive appositions to GnRH cells located at both levels examined. Thus the effect of estrogen on GnRH cells may be mediated in part by changes in the number of synaptic contacts.
