Related Experiment Video
Updated: Jul 28, 2026
Nasal Potential Difference to Quantify Trans-epithelial Ion Transport in Mice
Published on: July 4, 2018
Expression of the voltage-dependent chloride channel ClC-3 in human nasal tissue
T Oshima1, K Ikeda, M Furukawa
1Department of Otolaryngology, Tohoku University School of Medicine, Sendai, Japan. oshima@orl.med.tohoku.ac.jp
Abstract:
In the present study, ClC-3, one of the voltage-dependent chloride channels, was identified in human nasal tissue. In situ hybridization and immunohistochemical investigations demonstrated the localization of ClC-3 in the serous acini and ductal portions of submucosal nasal glands, which are the primary source of nasal secretion. Our data suggest that this channel contributes to nasal secretion via chloride transport. Its dysfunction might lead to abnormal nasal secretion in such pathological states as sinusitis.
More Related Videos
08:27Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
Published on: January 7, 2019
06:59A Fluorescence-Based Assay of Membrane Potential for High-Throughput Functional Study of Two Endogenous Ion Channels in Two Epithelial Cell Lines
Published on: June 22, 2022
Related Concept Videos
01:18Patch Clamp
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...
01:26Voltage-gated Ion Channels
Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several types of...