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Characterization of mouse GBP28 and its induction by exposure to cold
1Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
Objective:
To investigate whether the expression of the novel adipose tissue-specific protein GBP28 in adipose tissue and serum are altered in mice under a variety of conditions.
Design:
Mice were fed a high-fat diet for 4 weeks, fasted for 48 h or exposed at 4 degrees C.
Subjects:
C57BL/6J mouse, male, 4--6 weeks old.
Measurements:
GBP28 mRNA, GBP28 protein, blood glucose, insulin and fad pad weight of the mice.
Results:
We first confirmed that the mouse has GBP28 and its characteristics are the same as human GBP28. Serum concentration and mRNA levels of GBP28 significantly increased in the mice exposed to cold.
Conclusion:
GBP28 may play a role in homeostasis, regulating body temperature and basal metabolic rate in response to changing environmental conditions. International Journal of Obesity (2001) 25, 75-83