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Production of matrix metalloproteinase-9 in CaCO-2 cells in response to inflammatory stimuli

X Gan1, B Wong, S D Wright

  • 1Department of Lipid Biochemistry, Merck Research Laboratories, Rahway, NJ 07065, USA.

Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research
|March 13, 2001
PubMed

Insights

Matrix metalloproteinase-9 (MMP-9) is upregulated in intestinal epithelial cells by inflammatory cytokines, but not bacterial LPS. PPAR-gamma agonists may offer a therapeutic strategy for inflammatory bowel disease (IBD).

Area of Science:

  • Gastroenterology
  • Immunology
  • Molecular Biology

Background:

  • Matrix metalloproteinase-9 (MMP-9) is implicated in inflammatory bowel disease (IBD) pathogenesis.
  • The specific cellular origins of MMP-9 within inflamed IBD mucosa are not fully understood.

Purpose of the Study:

  • To investigate the cellular source and regulation of MMP-9 in intestinal epithelial cells.
  • To explore the potential therapeutic role of peroxisome proliferator-activated receptor-gamma (PPAR-gamma) agonists in IBD.

Main Methods:

  • MMP-9 expression and secretion were analyzed in CaCO-2 cells stimulated with various inflammatory mediators.
  • Quantitative analysis of MMP-9 mRNA and protein levels over time.
  • Assessment of the effect of rosiglitazone (a PPAR-gamma agonist) on MMP-9 secretion.

Main Results:

  • MMP-9 mRNA is expressed in CaCO-2 intestinal epithelial cells and its expression is significantly upregulated by interleukin-1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha) in a dose-dependent manner.
  • Bacterial lipopolysaccharide (LPS) did not induce MMP-9 expression, suggesting a requirement for inflammatory cytokine signaling.
  • IL-1beta-induced MMP-9 expression peaked at 16 hours, while earlier peaks were observed for monocyte chemoattractant protein-1 (MCP-1) and IL-8.
  • Rosiglitazone treatment significantly reduced MMP-9 secretion.

Conclusions:

  • Intestinal epithelial cells are a source of MMP-9, with expression induced by inflammatory cytokines.
  • PPAR-gamma activation may represent a viable therapeutic approach for managing MMP-9-related inflammation in IBD.

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