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[Relationship between oval cells and preneoplastic lesions induced by 2-acetylaminofluorene in rat liver]
1Department of Pathology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & School of Basic Medicine, Peking Union Medical College, Beijing 100005.
Objective:
To study the cellular origin of preneoplastic lesion of liver induced by the 2-acetylaminofluorene (2-AAF) in rat.
Methods:
Male fisher 344 rats were used for two different carcinogenesis models. Model 1: Rats were fed with regular diet containing 0.02% 2-AAF for totally 28 days, and subjected to a standard two thirds partial hepatectomy (2/3 PH) on the 7th day in order to stimulate the proliferation of oval cells. 3H thymidine (3H-Tdr) was administered intraperitoneally on the 12th day for selective labelling of oval cells. Model II: Rats were subjected to 2/3 PH and 3H-Tdr was administered intraperitoneally 22 hours after the operation for the selective labelling of hepatocytes. The rats were then fed with 2-AAF for totally 28 days after a 9 days recovery period from 2/3 PH. The relationship between preneoplastic lesion and selectively labelled oval cells, or hepatocytes, was traced by autoradiography.
Results:
Pathological examination and autoradiography denoted that the silver grains (labelled signals) were only formed in the preneoplastic cells in model I rather than the cells of model II animals.
Conclusion:
It is a direct evidence to demonstrate that the preneoplastic cells are mostly originated from the oval cells.