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[Correlation between human papillomavirus type and grading of cervical intraepithelial neoplasia]
1Department of Pathology, Beijing Friendship Hospital, Capital University of Medical Sciences, Beijing 100050.
Objective:
To disclose the correlation between human papillomavirus (HPV) type and grade of cervical intraepithelial lesion (CIN).
Methods:
PCR and in situ hybridization (ISH) techniques were used to detect the presence of HPV 6B/11, 16, 18 in 61 cases of CIN and 12 cases of cervical squamous cell carcinoma (SCC).
Results:
PCR showed HPV 6, 11 distributing mainly in low-grade squamous intraepithelial lesion (LSIL, 61.9%) and partly in CINII (20%), but not in CINIII and SCC. The frequency of HPV 16, 18 increased corresponding to the increasing of CIN grading and up to 83.3% in SCC. ISH showed HPV 6b/11, 16, 18 DNA hybridization intranuclear signal were diffusely distributed in LSIL and granular in appearance in episomal HPV. Similar signal were also seen in CINII infected by HPV 6b/11 and partly by HPV 16, 18, anyhow, CINIII, SCC and partly CINII HPV 16, 18 DNA hybridization were nongranular in appearance.
Conclusion:
low-grade SIL associated with "low-risk" types of HPV 6, 11 belonged to productive infection. CINIII and SCC associated with "high-risk" types of HPV 16, 18 belonged to integrated infection, while CINII belonged to either productive or integrated infection.