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Updated: Jul 18, 2026
Cell Population Analyses During Skin Carcinogenesis
Published on: August 21, 2013
[p53 gene mutations in mouse skin tumors induced by DMBA-TPA-MNNG]
1Department of Pathology, West China University of Medical Sciences, Chengdu 610041.
Objective:
To investigate the relationship between p53 gene mutations and mouse skin tumors induced by three-step carcinogenesis.
Methods:
The exons 5-8 of p53 gene were examined in 37 DMBA-TPA-MNNG induced mouse skin tumors [including 6 mice with papillomas, 15 mice having well differentiated squamous cell carcinomas (SCCI) and 16 mice with intermediately differentiated squamous cell carcinomas (SCC II)].
Results:
No p53 gene mutation was detected in the papilloma group, whereas 25.8% (8/31) of the SCC group had p53 gene mutations (4/15 of the SCC I mice and 4/16 of the SCC II mice). A total of 9 mutations were found in 8 mice with SCC, of which 7 were located in exon 8 and 7 were G-->A transitions.
Conclusion:
The p53 gene mutations occurred during the process of malignant transformation from papilloma to SCC induced by three step carcinogenesis.
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