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Related Experiment Videos

The telomeric dG(GT)4G sequence can adopt a parallel-stranded double helical conformation.

A K Shchyolkina1, O F Borisova, M A Livshits

  • 1Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow. annas@genome.eimb.relarn.ru

Journal of Biomolecular Structure & Dynamics
|March 14, 2001
PubMed
Summary

Oligonucleotides form a novel parallel-stranded intramolecular double helix with unusual base pairing. This finding is significant for understanding DNA structures in telomeres and gene regulation.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Background:

  • Repetitive DNA sequences like d(GT) are found in telomeres and gene regulatory regions.
  • Understanding the structural properties of these sequences is crucial for cell processes.

Purpose of the Study:

  • To investigate the structural properties of specific oligonucleotide sequences, hp-GT and hp-SGT.
  • To determine the formation of novel parallel-stranded (ps) intramolecular double helices.

Main Methods:

  • Optical techniques were employed to study oligonucleotide structures.
  • Molecular modeling with force field techniques was used to derive base pair configurations and conformational parameters.

Main Results:

  • Oligonucleotides hp-GT and hp-SGT formed a novel ps intramolecular double helix with purine-purine and pyrimidine-pyrimidine base pairing.

Related Experiment Videos

  • A quasi-two-state model validated helix-coil transition, yielding an enthalpy of -135 +/- 5 kJ/mol.
  • Analysis revealed G x G and T x T (or 4ST x T) base pair configurations.

    • Conclusions:

    • The repetitive d(GT) sequence exhibits unique conformational potential.
    • This potential may play a role in the regulation of cellular processes, including those involving telomeres and gene expression.