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Lipid-based microtubular drug delivery vehicles
N J Meilander1, X Yu, N P Ziats
1Biomaterials, Cell and Tissue Engineering Laboratory, Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH 44106-7207, USA.
Summary
This study presents a novel microtubule-hydrogel system for sustained bioactive agent release. The system demonstrates biocompatibility and preserves protein activity, offering a promising drug delivery solution.
Area of Science:
- Biomaterials Science
- Drug Delivery Systems
- Nanotechnology
Background:
- Self-assembling lipid microtubules offer potential for sustained bioactive agent release.
- Existing delivery systems may involve organic solvents, limiting applications.
Purpose of the Study:
- To develop and characterize a novel microtubule-hydrogel system for sustained release of bioactive agents.
- To evaluate the in vitro release profiles and in vivo biocompatibility of the system.
Main Methods:
- Lipid microtubules were self-assembled and loaded with agents under aqueous conditions.
- Microtubules were embedded in an agarose hydrogel for localized delivery.
- Protein release kinetics were studied using myoglobin, albumin, and thyroglobulin.
- In vitro cell culture and in vivo rodent implantation were used for biocompatibility assessment.
Main Results:
- Protein release rate was inversely proportional to molecular weight.
- Higher loading concentrations increased total mass released but not daily percentage.
- Released neurotrophic factor maintained bioactivity, inducing neurite extension in PC12 cells.
- The system exhibited no cytotoxicity to smooth muscle cells and no significant inflammatory response in vivo.
Conclusions:
- The microtubule-hydrogel system enables sustained release of bioactive agents without organic solvents.
- The system is biocompatible and preserves the activity of released proteins.
- This technology is suitable for applications requiring localized, sustained delivery of therapeutic agents.