Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Slow inhibitory potentials in the teleost Mauthner cell.

K Hatta1, N Ankri, D S Faber

  • 1Biologie Cellulaire et Moléculaire du Neurone, INSERM U261, Institut Pasteur, 25, rue du Dr Roux, 75724 Cedex 15, Paris, France.

Neuroscience
|March 14, 2001
PubMed
Summary

Researchers discovered new, long-lasting inhibitory synaptic events in zebrafish Mauthner cells. These slow inhibitory postsynaptic potentials (IPSPs) differ from fast glycinergic IPSPs and may be GABAergic, contributing to tonic inhibition and regulating neuronal excitability.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Discharge characteristics of steady-state high-density plasma source based on cascade arc discharge with hollow cathode.

The Review of scientific instruments·2022
Same author

Dietary Intake Is Associated with Occlusal Force Rather Than Number of Teeth in 80-y-Old Japanese.

JDR clinical and translational research·2019
Same author

Densities and biomasses of non-fossorial southern African Savanna rodents during the dry season.

Oecologia·2017
Same author

Initiation of early breeding in a population of Microtus townsendii (Rodentia) with the secondary plant compound 6-MBOA.

Oecologia·2017
Same author

Effects of live-trapping and toe-clipping on body weight of European and African rodent species.

Oecologia·2017
Same author

Longitudinal study of factors affecting taste sense decline in old-old individuals.

Journal of oral rehabilitation·2016

Area of Science:

  • Neuroscience
  • Cellular Biology
  • Synaptic Transmission

Background:

  • Mauthner cells are crucial for escape behaviors in fish.
  • Fast inhibitory postsynaptic potentials (IPSPs) mediated by glycine are well-characterized in Mauthner cells.
  • The full spectrum of inhibitory inputs to Mauthner cells remains incompletely understood.

Purpose of the Study:

  • To identify and characterize novel synaptic events in Mauthner cells.
  • To determine the properties and potential neurotransmitter mediation of these events.
  • To investigate the contribution of these events to Mauthner cell function and network activity.

Main Methods:

  • In vivo electrophysiological recordings from Mauthner cells in adult zebrafish and goldfish.
  • Utilized potassium chloride-filled electrodes for intracellular recordings.

Related Experiment Videos

  • Pharmacological manipulations with receptor antagonists and chloride loading.
  • Analysis of synaptic event kinetics, voltage dependence, and pharmacological profiles.
  • Autocorrelation analysis to study network rhythms.

    • Main Results:

    • A novel class of long-lasting inhibitory postsynaptic potentials (IPSPs) with decay time constants of 20–80 ms was identified.
    • These slow IPSPs are inhibitory, voltage-dependent, and pharmacologically distinct from fast glycinergic IPSPs, suggesting potential GABAergic mediation.
    • Amplitude distributions indicate synchronous release of multiple quantal units for slow events.
    • Autocorrelation analysis revealed distinct network rhythms (gamma-like) associated with fast and slow IPSPs, originating from different inhibitory networks.
    • Slow IPSPs contribute to tonic inhibition, shunting Mauthner cell excitability and responsiveness.

    Conclusions:

    • A new class of slow, potentially GABAergic, inhibitory postsynaptic potentials exists in Mauthner cells.
    • These slow IPSPs play a significant role in regulating Mauthner cell excitability through tonic inhibition.
    • The distinct properties and network origins of fast and slow IPSPs highlight complex inhibitory control mechanisms in Mauthner cells.
    • Developmental modifications or glycine receptor subtype assembly may influence fast IPSP kinetics.