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Virus-like particles in a case of acute hepatitis with human GB virus-C viraemia
1Medizinische Klinik und Poliklinik-Allgemeine Innere Medizin der Rheinischen, Friedrich Wilhelms Universität, Bonn, Germany.
Background/Aims:
It is still controversial whether the human GB virus-C can cause liver injury, as in situ demonstration of the virus is still inconclusive.
Methods:
Here we describe the case report of a patient with two episodes of severe acute hepatitis, who had a meticulous clinical, immunological and microbiological work-up, including human GB virus-C RNA detection by in situ hybridization and electron microscopy.
Results:
Human GB virus-C viraemia was found as the only potential cause of hepatitis in this patient. Furthermore, virus-like particles could be demonstrated in the cytoplasm of single hepatocytes by electron microscopy and human GB virus-C RNA was detected in the liver by in situ hybridization.
Conclusion:
The presence of human GB virus-C RNA in serum together with the demonstration of human GB virus-C RNA in the liver, favour acute human GB virus-C infection as the cause of liver injury in this patient. Thus virus-like particles and hepatic human GB virus-C RNA should be specifically looked for by electron microscopy and in situ hybridization, especially during the diagnostic work-up of patients with unexplained hepatitis.
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