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Related Experiment Videos

(Immuno)affinity chromatography: a versatile tool for fast and selective purification, concentration, isolation and

M M Rhemrev-Boom1, M Yates, M Rudolph

  • 1ResQ lab BV, Kolderveense Bovenboer 14, 7948 LV Nijeveen, The Netherlands. m.mrhemrevboom@freeler.nl

Journal of Pharmaceutical and Biomedical Analysis
|March 15, 2001
PubMed
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A novel solid phase support enhances (immuno)affinity separations with low non-specific adsorption and high ligand accessibility. This material enables selective isolation of compounds from complex samples on analytical and preparative scales.

Area of Science:

  • Biochemistry
  • Chromatography
  • Materials Science

Background:

  • Affinity-based separations leverage biomolecule selectivity for isolating compounds from complex matrices.
  • The performance of these techniques heavily relies on the properties of the solid phase support used for ligand immobilization.
  • Continuous research seeks new materials offering improved characteristics for enhanced separation efficiency.

Purpose of the Study:

  • To introduce and evaluate a new solid phase support for (immuno)affinity applications.
  • To demonstrate the material's capability for low non-specific adsorption and high ligand accessibility.
  • To showcase its utility in both analytical and preparative scale selective isolations.

Main Methods:

  • Development of a new solid phase support material.

Related Experiment Videos

  • Immobilization of ligands (antibodies, enzymes, receptors) onto the support.
  • Application in immunoaffinity for oestradiol and vitamin B12 determination.
  • Application in affinity chromatography for glucose detection via enzyme immobilization.
  • Production of an affinity column for xeno-oestrogenic activity screening using immobilized human oestrogen receptor.

    • Main Results:

    • The new support exhibits low non-specific adsorption and high ligand accessibility, leading to enhanced selectivity and capacity.
    • Successful selective isolations were performed on both analytical and preparative scales.
    • Demonstrated applications include the determination of oestradiol in serum and vitamin B12 in fermentation broth.
    • An enzyme reactor for glucose detection and an affinity column for xeno-oestrogen screening were successfully developed and tested.

    Conclusions:

    • The novel solid phase support is a promising material for diverse (immuno)affinity separation applications.
    • Its properties facilitate high selectivity and capacity, outperforming other chromatographic methods.
    • The material's stability and availability support its use in both research and industrial settings.