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Related Experiment Videos

Doping control for nandrolone using hair analysis.

P Kintz1, V Cirimele, V Dumestre-Toulet

  • 1Institut de Médecine Légale, Universite Louis Pasteur, 11 rue Humann, F-67000 Strasbourg, France. pascal.kintz@wanadoo.fr

Journal of Pharmaceutical and Biomedical Analysis
|March 15, 2001
PubMed
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A new method accurately quantifies nandrolone in human hair, crucial for detecting anabolic steroid use. This sensitive assay provides reliable results for forensic and clinical applications.

Area of Science:

  • Forensic Toxicology
  • Analytical Chemistry
  • Pharmacology

Background:

  • Nandrolone is an anabolic-androgenic steroid misused for performance enhancement.
  • Detecting nandrolone in biological matrices like hair is vital for doping control and forensic investigations.
  • Existing methods may lack the sensitivity or specificity required for hair analysis.

Purpose of the Study:

  • To develop and validate a sensitive, specific, and reproducible method for the quantitative determination of nandrolone in human hair.
  • To establish a reliable analytical technique for detecting nandrolone abuse through hair analysis.

Main Methods:

  • Hair sample preparation involved decontamination, solubilization in NaOH, and internal standard addition (nandrolone-d3).
  • Extraction utilized solid-phase (Isolute C18) and liquid-liquid (pentane) techniques.

Related Experiment Videos

  • Derivatization with MSTFA/NH4I/2-mercaptoethanol followed by Gas Chromatography-Mass Spectrometry (GC-MS) analysis.

    • Main Results:

    • The assay demonstrated high sensitivity, detecting 0.5 pg/mg of nandrolone in hair.
    • Linearity was established from 1 to 50 pg/mg with a correlation coefficient of 0.997.
    • Nandrolone was detected in hair samples from bodybuilders at concentrations ranging from 1 to 7.5 pg/mg.

    Conclusions:

    • The developed method is suitable for the precise quantification of nandrolone in human hair.
    • This technique offers a valuable tool for forensic toxicology and the detection of nandrolone abuse.