Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nanoengineered structures for holding and manipulating liposomes and cells.

C F Wilson1, G J Simpson, D T Chiu

  • 1Department of Chemistry, Stanford University, California 94305-5080, USA.

Analytical Chemistry
|March 16, 2001
PubMed
Summary

Researchers developed advanced nanoengineered holding pipets for precise single-cell and liposome manipulation. This breakthrough enables new research in cell biology and drug delivery systems.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Remote Monitoring Instruments in Palliative Care for People With Heart Failure: A Scoping Review.

Journal of pain and symptom management·2026
Same author

Safety and efficacy of encorafenib-cetuximab combination in <i>BRAF</i> <sup><i>V600E</i></sup> -mutated metastatic colorectal cancer: real-world evidence from the CONFIDENCE Spanish multicenter study.

ESMO real world data and digital oncology·2026
Same author

Temporal trends and prognostic impact of pre-hospital delay in ST-elevation myocardial infarction-20-year observational study from the SWEDEHEART registry.

European heart journal. Acute cardiovascular care·2025
Same author

Automatic milking unit preference of Holstein, Jersey, and Holstein-Jersey crossbred cows in a batch milking system.

Journal of dairy science·2025
Same author

Scaling Law for Epithelial Tissue Rheology.

Physical review letters·2025
Same author

The Surface-Topography Challenge: A Multi-Laboratory Benchmark Study to Advance the Characterization of Topography.

Tribology letters·2025

Area of Science:

  • Biotechnology
  • Cell Biology
  • Nanotechnology

Background:

  • Precise manipulation of single cells and liposomes is crucial for various biological and nanotechnological applications.
  • Existing methods often lack the stability and control required for delicate operations.

Purpose of the Study:

  • To fabricate and characterize novel nanoengineered holding pipets with enhanced stability and pressure control.
  • To demonstrate the utility of these pipets for single-cell and liposome manipulation in complex experimental settings.

Main Methods:

  • Fabrication of nanoengineered holding pipets featuring concave seating surfaces and fine pressure regulation.
  • Demonstration of pipet capabilities through three distinct experimental protocols: cell tagging and medium exchange, liposome transport and interrogation, and liposome electrofusion.

Related Experiment Videos

Main Results:

  • The nanoengineered pipets demonstrated exceptional stability in capturing, transporting, and releasing single cells and liposomes (1-12 microm).
  • Successful selective incorporation of carboxyrhodamine into single cells, followed by rapid medium exchange and laser-induced fluorescence (LIF) probing.
  • Stable manipulation and LIF interrogation of a single liposome during physical agitation, and selective electrofusion of paired liposomes.

Conclusions:

  • The developed nanoengineered holding pipets offer a versatile and stable platform for advanced single-cell and liposome manipulation.
  • This technology facilitates previously inaccessible research avenues, particularly in cellular assays and nanomedicine.
  • The system's fine control and stability are key to its utility in demanding biological experiments.