Recording depth of the heterodyne laser interferometer for cochlear vibration measurement

Measurement of the cochlear partition vibration as a function of the optical-axis (z-axis) position in the gerbil cochlea showed that the velocity distributes over a range of more than 300 microm, which is larger than the thickness of the cochlear partition. This finding suggests that the recording depth (RD) of the heterodyne interferometer probably is not as small as reported in the literature. In the current experiment, the RD of the heterodyne laser interferometer was studied by measuring the velocity of a vibrating mirror as a function of the z-axis position. Results demonstrate that the optical sectioning characteristic, measured by the intensity of the reflected laser beam as a function of the z-axis position, is not able to correctly estimate the RD of the heterodyne interferometer: the RD is much larger than optical sectioning, indicating a poor spatial resolution along the z axis.