Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Aptamers: selected oligonucleotides for therapy.

J J Toulmé1

  • 1INSERM U.386, Université Victor Segalen, Bordeaux, France. jean-jacques.toulme@bordeaux.inserm.fr

Current Opinion in Molecular Therapeutics
|March 16, 2001
PubMed
Summary

In vitro selection identifies novel nucleic acid aptamers for understanding diseases. These aptamers can be modified for therapeutic applications, including gene therapy and drug design.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A method to select chemically modified aptamers directly.

Antisense & nucleic acid drug development·2002
Same author

New ways of initiating translation in eukaryotes.

Molecular and cellular biology·2001
Same author

Gel renaturation assay for ribonucleases.

Methods in enzymology·2001
Same author

RNA and N3'-->P5' kissing aptamers targeted to the trans-activation responsive (TAR) RNA of the human immunodeficiency virus-1.

Nucleosides, nucleotides & nucleic acids·2001
Same author

Modulation of RNA function by oligonucleotides recognizing RNA structure.

Progress in nucleic acid research and molecular biology·2001
Same author

DNA aptamers selected against the HIV-1 RNase H display in vitro antiviral activity.

Biochemistry·2001

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • Vast libraries of random nucleic acids can be explored using in vitro selection.
  • Selected RNA and DNA sequences, known as aptamers, offer insights into molecular interactions relevant to human diseases.

Purpose of the Study:

  • To explore the potential of in vitro selection for discovering novel aptamers.
  • To investigate the therapeutic applications of chemically modified aptamers.

Main Methods:

  • In vitro selection of RNA and DNA aptamers from large random libraries.
  • Chemical modification of selected aptamers to enhance stability and functionality.
  • Evaluation of aptamer activity in complex biological media and in vivo.

Main Results:

  • Identification of specific aptamers capable of interacting with disease-related targets.
  • Demonstration of improved aptamer efficiency and stability through chemical modifications.
  • Evidence of in vivo aptamer activity, suggesting therapeutic potential.

Conclusions:

  • In vitro selection is a powerful tool for discovering functional nucleic acid aptamers.
  • Chemically modified aptamers hold promise for advanced diagnostics and therapeutics.
  • Aptamers represent a novel class of molecules for gene therapy and drug design.

Related Experiment Videos