Total urine antioxidant capacity

Area of Science:

• Biochemistry
• Nephrology
• Clinical Chemistry

Background:

• Total antioxidant capacity (TAC) in body fluids can assess antioxidant defenses in inflammatory conditions.
• Urine analysis offers a non-invasive method to evaluate systemic oxidative stress.

Purpose of the Study:

• To determine urine total antioxidant capacity (TAC) using the ABTS assay.
• To compare antioxidant profiles in urine from control subjects and patients with hypertension and renal diseases.
• To investigate correlations between urine TAC and other biomarkers.

Main Methods:

• Urine samples from controls and patients (hypertension, acute renal failure, chronic renal disease) were analyzed.
• ABTS antioxidant assay was employed to measure TAC.
• Fluorescence markers for advanced glycosylation end products (AGE) and di-tyrosine (di-tyr), alongside protein, uric acid, and creatinine, were quantified.

Main Results:

• Urine TAC, assessed by lag time and area under the curve (AUC) in the ABTS assay, correlated with protein and uric acid levels.
• This correlation was absent in patients with acute renal failure (ARF).
• Correlations were observed between TAC parameters and AGE/di-tyrosine fluorescence.

Conclusions:

• The primary urine antioxidants in ARF patients differ from those in patients with hypertension and chronic nephropathies.
• The ABTS assay is a practical tool for evaluating urine antioxidant content.
• Urine TAC analysis can help differentiate renal disease types based on antioxidant profiles.