Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Otx genes in brain morphogenesis.

D Acampora1, M Gulisano, V Broccoli

  • 1International Institute of Genetics and Biophysics, CNR, Via G. Marconi 12, 80125 Naples, Italy.

Progress in Neurobiology
|March 16, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Assessing value of contrast-enhanced ultrasound vs. conventional transthoracic ultrasound in improving diagnostic yield of percutaneous needle biopsy of peripheral lung lesions.

European review for medical and pharmacological sciences·2021
Same author

Ultrasound-guided fine needle aspiration biopsy (FNAB) demonstrating upper distal extremity metastasis of lung adenocarcinoma: a case report and review of the literature.

European review for medical and pharmacological sciences·2021
Same author

Applying science to pressing conservation needs for penguins.

Conservation biology : the journal of the Society for Conservation Biology·2019
Same author

Genetic Otx2 mis-localization delays critical period plasticity across brain regions.

Molecular psychiatry·2017
Same author

Gene therapy approaches in the non-human primate model of Parkinson's disease.

Journal of neural transmission (Vienna, Austria : 1996)·2017
Same author

Evidence of immunocompetence reduction induced by cadmium exposure in honey bees (Apis mellifera).

Environmental pollution (Barking, Essex : 1987)·2016
Same journal

Deletion of Fbxo25 causes excessive repetitive behavior, impaired recognition memory, reduced dendritic complexity, and aberrant protein expression in mice.

Progress in neurobiology·2026
Same journal

Neuropsychiatric Disease Mechanisms and Interventions. from 22q11.2 Deletion Syndrome Experimental Studies.

Progress in neurobiology·2026
Same journal

REM sleep as a dummy-model of the world: A theoretical framework.

Progress in neurobiology·2026
Same journal

CA3 transiently modulates spatial representation in CA1.

Progress in neurobiology·2026
Same journal

Love, death, and oxytocin: In memory of Larry Young.

Progress in neurobiology·2026
Same journal

Assessing peripheral oxytocin and cortisol levels and epigenetic variations of oxytocin receptor and glucocorticoid receptor genes in school-aged preterm-born children.

Progress in neurobiology·2026
See all related articles

The Otx genes are crucial for vertebrate brain development, controlling anterior neural patterning. Functional equivalence between Drosophila otd and mouse Otx genes suggests conserved roles in brain evolution.

Area of Science:

  • Developmental Biology
  • Neuroscience
  • Evolutionary Biology

Background:

  • Vertebrate brain morphogenesis relies on gene homologues from Drosophila, particularly transcription factors like the orthodenticle (otd) group.
  • The Drosophila otd gene and its vertebrate counterparts, Otx1 and Otx2, are key in anterior neural patterning and segmentation.

Purpose of the Study:

  • To investigate the conserved functions of Otx genes in brain development by generating mouse models.
  • To test the hypothesis of a conserved control system for anterior brain patterning distinct from HOX genes.

Main Methods:

  • Generation of mouse models with inactivated Otx1 and Otx2 genes, reciprocal gene replacements, and replacement with Drosophila otd.
  • Analysis of phenotypic defects in mutant mice, including neurological, hormonal, and developmental abnormalities.

Related Experiment Videos

  • Functional assessment of Otx genes through reciprocal gene replacement experiments between Drosophila and mouse.

    • Main Results:

    • Otx1 knockout mice exhibit epilepsy and sensory/hormonal defects. Otx2 knockout mice are embryonically lethal, failing in gastrulation and anterior neural plate specification.
    • A minimum threshold of Otx1/Otx2 protein levels is essential for correct midbrain-hindbrain boundary positioning.
    • Reciprocal gene replacement confirmed functional equivalence between Drosophila otd and mouse Otx1/Otx2 in vivo.

    Conclusions:

    • Otx genes play a critical role in the specification, regionalization, and terminal differentiation of the rostral central nervous system (CNS).
    • The functional conservation of Otx genes suggests their regulatory control has influenced vertebrate brain morphogenesis and evolution.