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Updated: Aug 13, 2026
A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples
Published on: October 2, 2009
Covalent coupling of asparagus pea and tomato lectins to poly(lactide) microspheres
M J Montisci1, G Giovannuci, D Duchêne
1Pharmaceutical Technology and Biopharmaceutics Department, UMR CNRS 8612, Faculty of Pharmacy, University of Paris-South, 5 Rue J.B. Clement, 92296 Châtenay-Malabry Cedex, Paris, France.
Abstract:
Lectin-poly(lactide) microsphere conjugates specifically designed for oral administration were prepared and their activity and specificity in presence of mucus were characterized. The presence of hydroxyl or amino groups suitable for covalent coupling of lectins by the glutaraldehyde method at the surface of the microspheres have been ensured by preparing the particles in presence either of poly(vinyl alcohol) (PVA) or bovine serum albumin (BSA). Tomato and asparagus pea lectins could be covalently attached to these particles (1.0-1.3 mg/m(2) of particles). The conjugates demonstrated a 4-10 fold increase in their interactions with mucus compared to control particles. Moreover, the sugar specificity of the lectins was maintained.
