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Updated: Aug 12, 2026

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA
Published on: September 10, 2013
The ins and outs of nucleosome assembly
1Institut Curie, Research section, UMR 218 du Centre National de la Recherche Scientifique (CNRS), 75248 Paris 05, Cedex, France. jill.mello@curie.fr
Abstract:
De novo nucleosome assembly coupled to DNA replication and repair in vitro involves the histone chaperone chromatin assembly factor 1 (CAF-1). Recent studies support a model in which CAF-1 can be targeted to newly synthesized DNA through a direct interaction with proliferating cell nuclear antigen (PCNA) and can act synergistically with a newly identified histone chaperone. Insights have also been obtained into mechanisms by which this CAF-1-dependent pathway can establish a repressed chromatin state.
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