Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Chromosomal boundaries in S. cerevisiae.

X Bi1, J R Broach

  • 1Department of Biochemistry, University of Nebraska, Lincoln 68588-0664, USA. xbi@unlserve.edu

Current Opinion in Genetics & Development
|March 16, 2001
PubMed
Summary

Chromatin boundary elements, known as heterochromatin barriers in yeast, block the spread of silent chromatin. These DNA sequences recruit proteins that prevent nucleosome assembly, disrupting chromatin structure.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular detection and identification of <i>Enteromonas</i> species in human and animal hosts using polymerase chain reaction and DNA sequencing.

MethodsX·2024
Same author

Molecular screening of <i>Entamoeba</i> spp. (<i>E. histolytica, E. dispar, E. coli</i>, and <i>E. hartmanni</i>) and <i>Giardia</i> intestinalis using PCR and sequencing.

MethodsX·2023
Same author

A novel nonsense mutation of PNLDC1 associated with male infertility due to oligo-astheno-teratozoospermia in a consanguineous Chinese family.

QJM : monthly journal of the Association of Physicians·2023
Same author

[Bioinformatics analysis and prokaryotic expression of <i>Strongyloides stercoralis</i> serine protease inhibitor 1].

Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control·2023
Same author

Case report: human early embryonic arrest in a consanguineous Chinese family caused by a novel missense variant of PADI6.

QJM : monthly journal of the Association of Physicians·2023
Same author

[Expression of CD47 and its ligands in pregnant mice infected with <i>Toxoplasma gondii</i> during pregnancy].

Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control·2023

Area of Science:

  • Molecular Biology
  • Genetics
  • Epigenetics

Background:

  • Chromatin boundary elements (insulators) define gene expression domains in metazoans.
  • Similar DNA sequences, termed 'heterochromatin barriers,' function in Saccharomyces cerevisiae.
  • These barriers prevent the spread of transcriptionally silent chromatin, the yeast equivalent of heterochromatin.

Purpose of the Study:

  • To investigate the mechanism by which heterochromatin barriers function in yeast.
  • To understand how these barriers disrupt the spread of silent chromatin.

Main Methods:

  • Analysis of DNA sequences with boundary element properties in yeast.
  • Identification of regulatory proteins binding to these sequences.
  • Hypothesizing the recruitment of protein complexes.

Main Results:

  • Heterochromatin barriers in yeast lack sequence homology.
  • These barriers contain multiple binding sites for various regulatory proteins.
  • Barriers are proposed to recruit protein complexes.

Conclusions:

  • Yeast heterochromatin barriers function by recruiting regulatory proteins.
  • Recruited proteins likely preclude nucleosome assembly.
  • This disruption of nucleosome arrays prevents the spread of silent chromatin.

Related Experiment Videos